stempro ® osteogenic differentiation kit  (Thermo Fisher)

Journal: Cell and Tissue Research

Article Title: Minor salivary gland stem cells: a comparative study of the biological properties under clinical-grade culture conditions

doi: 10.1007/s00441-023-03789-z

Figure Lengend Snippet: a – f Evaluation by real-time PCR of expression of markers for osteogenic (ALP, BMP-2), chondrogenic (SOX-9, ACAN), and adipogenic (PPAR-γ2, LPL) differentiation in mSG-SCs after several subculturing in three different media (CCM, StemMacs, and StemPro). Mean values ​​are recorded (± constant deviations, SD) ( n = 3/culture medium, repeating biological experiments three times with two technical samples each). Asterisks indicate the statistically significant differences (* p < 0.05; ** p < 0.01) between the individual culture media in each passage, while small a indicates the differences from the original expression (p.2) in each medium (i.e., at p.6 and p.10 relative to p.2)

Article Snippet: For osteogenic and chondrogenic differentiation, 300,000 cells were seeded in 6 well plates until reaching confluency, and the medium was changed (StemPro osteogenic and StemPro chondrogenic differentiation kits; Life Technologies).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Subculturing Assay

Journal: Cell and Tissue Research

Article Title: Minor salivary gland stem cells: a comparative study of the biological properties under clinical-grade culture conditions

doi: 10.1007/s00441-023-03789-z

Figure Lengend Snippet: a – c Real-time PCR analysis of the expression of osteogenic marker genes (ALP, BMP-2, BGLAP) after corresponding induction mSG-SCs from passages p.2 (early), p.6 (intermediate), and p.10 (late) grown in the three different media (CCM, StemMacs, and StemPro). Values ​​are mean (± SD) of three ( n = 3) different experiments with two repetitions each. The asterisks above the horizontal double arrows indicate the statistically significant differences (* p < 0.05; ** p < 0.01; ns = non-significant) in each passage for each culture medium (CCM, StemMacs, StemPro), during induction (osteogenic/coarse) for 7 and 14 days (D0, D7, and D14). d – f '' Histochemical staining with alizarin red in three different cultures in CCM, StemPro, and StemMacs. In initial (p.2), medium (p.6), and final (p.10) stage passage of the experiment. g Spectrophotometric evaluation of in vitro bioremediation (measurement of bound alizarin) expressed as mean mmol alizarin from three independent experiments with three replications each, in the culture of mSG-SCs developed in principle in one of the three different media, namely CCM, StemPro, and StemMacs and then in each passage (initial p.3, mean p.7, and final p.11) induction of differentiation with a special culture medium for 21 days. The statistics comparisons between passages and culture media were made by analysis two-way ANOVA and Tukey’s post-hoc tests

Article Snippet: For osteogenic and chondrogenic differentiation, 300,000 cells were seeded in 6 well plates until reaching confluency, and the medium was changed (StemPro osteogenic and StemPro chondrogenic differentiation kits; Life Technologies).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Marker, Staining, In Vitro

Journal: Cell and Tissue Research

Article Title: Minor salivary gland stem cells: a comparative study of the biological properties under clinical-grade culture conditions

doi: 10.1007/s00441-023-03789-z

Figure Lengend Snippet: Real-time PCR analysis of the expression of chondrogenic differentiation markers, including a ACAN and b SOX-9 after corresponding induction mSG-SCs from passages p.2 (early), p.6 (intermediate),and p.10 (late) grown in the three different media (CCM, StemMacs and StemPro). Values ​​are mean (± SD) of three ( n = 3) different experiments with two repetitions each. The asterisks above the horizontal double arrows indicate the statistically significant differences (* p < 0.05; ** p < 0.01; ns = non-significant) in each passage for each culture medium (CCM, StemMacs, StemPro), during induction (osteogenic/coarse) for 7 and 14 days (D0, D7, and D14)

Article Snippet: For osteogenic and chondrogenic differentiation, 300,000 cells were seeded in 6 well plates until reaching confluency, and the medium was changed (StemPro osteogenic and StemPro chondrogenic differentiation kits; Life Technologies).

Techniques: Real-time Polymerase Chain Reaction, Expressing